41 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Cumberland township | 1 | 6,040 | 6,370 | -0.87% | 158 | 38.24 | Township |
| Jefferson township | 2 | 5,017 | 5,202 | -0.54% | 219 | 22.94 | Township |
| Jefferson township | 3 | 3,926 | 3,757 | 0.9% | 117 | 33.55 | Township |
| Waynesburg | 4 | 3,552 | 3,991 | -2.42% | 4,412 | 0.81 | Borough |
| Jefferson township | 5 | 2,380 | 2,364 | 0.25% | 150 | 15.91 | Township |
| Fairdale | 6 | 2,352 | 2,358 | 0.86% | 1,630 | 1.44 | CDP |
| Jefferson township | 7 | 2,201 | 2,348 | -1.03% | 102 | 21.54 | Township |
| Dunkard township | 8 | 2,029 | 2,167 | -1.17% | 64 | 31.57 | Township |
| Jefferson township | 9 | 1,892 | 2,005 | -1.15% | 94 | 20.03 | Township |
| Jefferson township | 10 | 1,828 | 1,917 | -1.08% | 74 | 24.66 | Township |
| Aleppo township | 11 | 1,745 | 1,824 | -0.8% | 980 | 1.78 | Township |
| Jefferson township | 12 | 1,383 | 1,428 | -0.72% | 34 | 41.22 | Township |
| Perry township | 13 | 1,321 | 1,391 | -0.83% | 44 | 30.23 | Township |
| Jefferson township | 14 | 1,112 | 1,120 | -0.18% | 49 | 22.63 | Township |
| Center township | 15 | 1,063 | 1,110 | -0.37% | 22 | 48.95 | Township |
| Nemacolin | 16 | 944 | 943 | 0% | 843 | 1.12 | CDP |
| Bobtown | 17 | 786 | 618 | 0.26% | 1,276 | 0.62 | CDP |
| Deemston | 18 | 736 | 753 | -0.41% | 77 | 9.59 | Borough |
| Richhill township | 19 | 719 | 769 | -0.96% | 13 | 54.28 | Township |
| Mount Morris | 20 | 676 | 571 | -0.59% | 254 | 2.66 | CDP |
| Jefferson | 21 | 651 | 656 | -0.15% | 1,074 | 0.61 | Borough |
| Dry Tavern | 22 | 609 | 551 | -0.98% | 450 | 1.35 | CDP |
| Whiteley township | 23 | 528 | 568 | -1.31% | 17 | 31.66 | Township |
| West Waynesburg | 24 | 455 | 431 | 0.22% | 2,177 | 0.21 | CDP |
| Mather | 25 | 425 | 495 | -4.49% | 484 | 0.88 | CDP |
| Aleppo township | 26 | 400 | 423 | -1.23% | 14 | 27.95 | Township |
| Carmichaels | 27 | 394 | 431 | -1.75% | 2,388 | 0.17 | Borough |
| Rices Landing | 28 | 387 | 419 | -1.53% | 487 | 0.79 | Borough |
| Greene township | 29 | 383 | 397 | -1.03% | 21 | 18.51 | Township |
| Jefferson township | 30 | 352 | 359 | -0.56% | 14 | 24.36 | Township |
| Springhill township | 31 | 299 | 321 | -0.99% | 14 | 21.95 | Township |
| Greensboro | 32 | 245 | 265 | -1.61% | 2,269 | 0.11 | Borough |
| Rogersville | 33 | 235 | 330 | -0.42% | 607 | 0.39 | CDP |
| Jefferson | 34 | 233 | 252 | -1.69% | 1,239 | 0.19 | Borough |
| Crucible | 35 | 222 | 395 | -13.28% | 193 | 1.15 | CDP |
| Gilmore township | 36 | 220 | 237 | -1.35% | 10 | 23.04 | Township |
| Wind Ridge | 37 | 189 | 179 | -0.53% | 59 | 3.19 | CDP |
| Clarksville | 38 | 180 | 194 | -1.64% | 1,856 | 0.10 | Borough |
| Brave | 39 | 171 | 205 | -1.16% | 303 | 0.56 | CDP |
| Mapletown | 40 | 103 | 113 | -0.96% | 286 | 0.36 | CDP |
| New Freeport | 41 | 72 | 53 | -4% | 127 | 0.57 | CDP |
Cumberland township is the largest city in Greene County, with a population of 6,040.
New Freeport is the smallest city in Greene County.
There are 41 cities in Greene County, Pennsylvania.