19 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Owego town | 1 | 18,898 | 18,750 | 0.3% | 181 | 104.17 | Town |
| Barton town | 2 | 8,329 | 8,567 | -0.45% | 140 | 59.42 | Town |
| Candor town | 3 | 4,960 | 5,138 | -0.36% | 52 | 94.99 | Town |
| Owego | 4 | 4,396 | 3,645 | 3.53% | 1,725 | 2.55 | Village |
| Tioga town | 5 | 4,276 | 4,430 | -0.56% | 73 | 58.44 | Town |
| Waverly | 6 | 4,205 | 4,367 | -0.76% | 1,841 | 2.28 | Village |
| Newark Valley town | 7 | 3,496 | 3,635 | -0.48% | 69 | 50.37 | Town |
| Tioga Terrace | 8 | 2,935 | 2,367 | 7.16% | 2,929 | 1 | CDP |
| Spencer town | 9 | 2,855 | 2,963 | -0.56% | 58 | 49.12 | Town |
| Apalachin | 10 | 2,662 | 2,148 | 3.98% | 926 | 2.88 | CDP |
| Nichols town | 11 | 2,299 | 2,344 | -0.09% | 68 | 33.78 | Town |
| Berkshire town | 12 | 1,480 | 1,479 | 0.41% | 49 | 30.29 | Town |
| Richford town | 13 | 1,020 | 1,042 | 0.1% | 27 | 38.19 | Town |
| Waverly town | 14 | 947 | 965 | -0.32% | 8 | 125.11 | Town |
| Newark Valley | 15 | 881 | 935 | -1.23% | 892 | 0.99 | Village |
| Candor | 16 | 744 | 786 | -1.06% | 1,706 | 0.44 | Village |
| Spencer | 17 | 683 | 725 | -1.16% | 660 | 1.03 | Village |
| Nichols | 18 | 446 | 471 | -1.11% | 861 | 0.52 | Village |
| West Danby | 19 | 276 | 216 | 6.98% | 202 | 1.37 | CDP |
Owego town is the largest city in Tioga County, with a population of 18,898.
West Danby is the smallest city in Tioga County.
There are 19 cities in Tioga County, New York.