49 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Oneonta | 1 | 16,852 | 13,049 | 4.73% | 3,864 | 4.36 | City |
| Oneonta town | 2 | 4,901 | 5,058 | -0.73% | 149 | 32.91 | Town |
| Unadilla town | 3 | 4,016 | 4,105 | -0.52% | 87 | 46.27 | Town |
| Otsego town | 4 | 3,553 | 3,635 | -0.67% | 66 | 53.89 | Town |
| Milford town | 5 | 2,703 | 2,820 | -0.95% | 59 | 46.12 | Town |
| Otego town | 6 | 2,642 | 2,749 | -1.01% | 58 | 45.63 | Town |
| Laurens town | 7 | 2,273 | 2,303 | -0.35% | 54 | 42.05 | Town |
| Worcester town | 8 | 2,028 | 2,107 | -0.88% | 43 | 46.71 | Town |
| Richfield town | 9 | 2,024 | 2,060 | -0.44% | 66 | 30.86 | Town |
| Hartwick town | 10 | 1,918 | 1,946 | -0.36% | 48 | 40.09 | Town |
| Edmeston town | 11 | 1,874 | 1,902 | -0.37% | 42 | 44.30 | Town |
| Middlefield town | 12 | 1,842 | 1,877 | -0.49% | 29 | 63.33 | Town |
| Cooperstown | 13 | 1,826 | 1,853 | -0.27% | 1,117 | 1.64 | Village |
| Maryland town | 14 | 1,729 | 1,750 | -0.35% | 33 | 52.41 | Town |
| Morris town | 15 | 1,706 | 1,728 | -0.35% | 44 | 39.07 | Town |
| Butternuts town | 16 | 1,633 | 1,656 | -0.37% | 30 | 53.83 | Town |
| Springfield town | 17 | 1,311 | 1,336 | -0.46% | 31 | 42.89 | Town |
| Pittsfield town | 18 | 1,292 | 1,310 | -0.31% | 34 | 37.98 | Town |
| Cherry Valley town | 19 | 1,208 | 1,233 | -0.49% | 30 | 40.38 | Town |
| Unadilla | 20 | 1,080 | 1,097 | -0.28% | 1,041 | 1.04 | Village |
| Richfield Springs | 21 | 1,079 | 1,098 | -0.37% | 1,071 | 1.01 | Village |
| New Lisbon town | 22 | 1,058 | 1,079 | -0.47% | 24 | 44.36 | Town |
| Burlington town | 23 | 1,022 | 1,041 | -0.49% | 23 | 44.89 | Town |
| Plainfield town | 24 | 905 | 923 | -0.55% | 31 | 29.51 | Town |
| Otego | 25 | 899 | 934 | -0.77% | 776 | 1.16 | Village |
| Exeter town | 26 | 825 | 843 | -0.6% | 26 | 32.08 | Town |
| Westford town | 27 | 796 | 808 | -0.38% | 24 | 33.85 | Town |
| Roseboom town | 28 | 657 | 684 | -0.9% | 20 | 33.41 | Town |
| Worcester | 29 | 638 | 802 | -4.78% | 74 | 8.56 | CDP |
| Edmeston | 30 | 553 | 543 | -1.25% | 127 | 4.34 | CDP |
| Morris | 31 | 517 | 521 | -0.19% | 693 | 0.75 | Village |
| Hartwick | 32 | 507 | 539 | -1.55% | 147 | 3.46 | CDP |
| Cherry Valley | 33 | 472 | 481 | -0.42% | 925 | 0.51 | Village |
| Schenevus | 34 | 471 | 556 | -1.05% | 458 | 1.03 | CDP |
| Milford | 35 | 398 | 402 | -0.25% | 939 | 0.42 | Village |
| Springfield Center | 36 | 375 | 392 | 7.14% | 78 | 4.81 | CDP |
| Decatur town | 37 | 367 | 371 | -0.27% | 18 | 20.56 | Town |
| Hartwick Seminary | 38 | 364 | 524 | 7.06% | 186 | 1.96 | CDP |
| Gilbertsville | 39 | 324 | 329 | -0.31% | 323 | 1 | Village |
| Wells Bridge | 40 | 305 | 151 | 7.02% | 337 | 0.90 | CDP |
| Fly Creek | 41 | 271 | 333 | 7.11% | 165 | 1.65 | CDP |
| East Worcester | 42 | 261 | 231 | 6.97% | 104 | 2.51 | CDP |
| Laurens | 43 | 215 | 217 | 0% | 1,680 | 0.13 | Village |
| Burlington Flats | 44 | 211 | 137 | 7.11% | 92 | 2.30 | CDP |
| Garrattsville | 45 | 188 | 149 | 7.43% | 132 | 1.43 | CDP |
| Portlandville | 46 | 145 | 342 | 7.41% | 630 | 0.23 | CDP |
| Mount Vision | 47 | 138 | 156 | 7.81% | 91 | 1.51 | CDP |
| Westford | 48 | 110 | 73 | 6.8% | 67 | 1.65 | CDP |
| Schuyler Lake | 49 | 102 | 106 | 6.25% | 32 | 3.24 | CDP |
Oneonta is the largest city in Otsego County, with a population of 16,852.
Schuyler Lake is the smallest city in Otsego County.
There are 49 cities in Otsego County, New York.