19 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Gallatin | 1 | 1,873 | 1,825 | 0.54% | 682 | 2.75 | City |
| Jamesport township | 2 | 1,122 | 1,092 | 0.81% | 33 | 34.28 | Township |
| Colfax township | 3 | 668 | 642 | 1.21% | 19 | 35.54 | Township |
| Jamesport | 4 | 571 | 560 | 0.35% | 1,018 | 0.56 | City |
| Jefferson township | 5 | 477 | 464 | 0.85% | 13 | 35.70 | Township |
| Lake Viking | 6 | 383 | 355 | -1.79% | 49 | 7.88 | CDP |
| Pattonsburg | 7 | 343 | 333 | 0.59% | 566 | 0.61 | City |
| Grand River township | 8 | 304 | 294 | 1% | 6 | 52.55 | Township |
| Marion township | 9 | 267 | 257 | 1.14% | 6 | 45.87 | Township |
| Winston | 10 | 239 | 229 | 0.84% | 776 | 0.31 | Village |
| Monroe township | 11 | 190 | 183 | 1.06% | 5 | 36.24 | Township |
| Altamont | 12 | 173 | 167 | 0.58% | 676 | 0.26 | Village |
| Harrison township | 13 | 157 | 153 | 0.64% | 7 | 21.57 | Township |
| Coffey | 14 | 153 | 150 | 0.66% | 736 | 0.21 | City |
| McFall | 15 | 120 | 119 | 0% | 391 | 0.31 | City |
| Jameson | 16 | 71 | 70 | 0% | 317 | 0.22 | Town |
| Avalon | 17 | 59 | 51 | 7.27% | 79 | 0.75 | CDP |
| Brimson | 18 | 47 | 47 | 0% | 540 | 0.09 | Village |
| Lock Springs | 19 | 39 | 38 | 0% | 315 | 0.12 | Village |
Gallatin is the largest city in Daviess County, with a population of 1,873.
Lock Springs is the smallest city in Daviess County.
There are 19 cities in Daviess County, Missouri.