20 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Neodesha | 1 | 2,154 | 2,259 | -0.97% | 1,533 | 1.41 | City |
| Fredonia | 2 | 2,028 | 2,140 | -1.07% | 831 | 2.44 | City |
| Neodesha township | 3 | 571 | 604 | -1.89% | 17 | 34.08 | Township |
| Center township | 4 | 491 | 520 | -2% | 12 | 39.39 | Township |
| Buffalo township | 5 | 425 | 432 | 0% | 12 | 34.14 | Township |
| Buffalo township | 6 | 425 | 452 | -1.39% | 11 | 40.43 | Township |
| Colfax township | 7 | 375 | 392 | -1.57% | 8 | 47.34 | Township |
| Altoona | 8 | 340 | 347 | -0.29% | 637 | 0.53 | City |
| Verdigris township | 9 | 267 | 276 | -1.48% | 7 | 39.52 | Township |
| Newark township | 10 | 215 | 232 | -2.27% | 6 | 35.71 | Township |
| Buffalo | 11 | 208 | 217 | -0.95% | 654 | 0.32 | City |
| Talleyrand township | 12 | 208 | 216 | -0.95% | 6 | 35.42 | Township |
| Guilford township | 13 | 160 | 164 | -0.62% | 5 | 35.53 | Township |
| Pleasant Valley township | 14 | 157 | 168 | -2.48% | 3 | 47.18 | Township |
| Prairie township | 15 | 110 | 113 | -0.9% | 4 | 29.97 | Township |
| Buffalo township | 16 | 92 | 93 | 0% | 2 | 43.29 | Township |
| Duck Creek township | 17 | 76 | 80 | -2.56% | 2 | 35.74 | Township |
| Benedict | 18 | 68 | 72 | -1.45% | 466 | 0.15 | City |
| New Albany | 19 | 57 | 57 | 0% | 308 | 0.18 | City |
| Webster township | 20 | 37 | 38 | 0% | 1 | 29.51 | Township |
Neodesha is the largest city in Wilson County, with a population of 2,154.
Webster township is the smallest city in Wilson County.
There are 20 cities in Wilson County, Kansas.