23 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| St. John | 1 | 1,137 | 1,221 | -1.47% | 585 | 1.94 | City |
| Stafford township | 2 | 1,021 | 1,084 | -1.35% | 28 | 36.04 | Township |
| St. John township | 3 | 927 | 1,002 | -1.8% | 26 | 36.29 | Township |
| Stafford | 4 | 903 | 957 | -1.2% | 987 | 0.92 | City |
| Farmington township | 5 | 480 | 516 | -1.84% | 13 | 36.13 | Township |
| Macksville | 6 | 442 | 469 | -1.12% | 443 | 1 | City |
| Ohio township | 7 | 333 | 360 | -1.77% | 9 | 36.52 | Township |
| Hayes township | 8 | 157 | 162 | -0.63% | 4 | 36.02 | Township |
| North Seward township | 9 | 129 | 137 | -1.53% | 4 | 35.94 | Township |
| Douglas township | 10 | 98 | 104 | -2% | 3 | 36.05 | Township |
| Hudson | 11 | 89 | 93 | -1.11% | 712 | 0.13 | City |
| Byron township | 12 | 57 | 62 | -1.72% | 2 | 36.31 | Township |
| West Cooper township | 13 | 57 | 62 | -1.72% | 2 | 35.92 | Township |
| Cleveland township | 14 | 55 | 53 | 1.85% | 2 | 36.15 | Township |
| York township | 15 | 54 | 56 | 0% | 2 | 35.88 | Township |
| Richland township | 16 | 42 | 46 | -2.33% | 1 | 36.18 | Township |
| East Cooper township | 17 | 41 | 43 | -2.38% | 1 | 35.67 | Township |
| South Seward township | 18 | 41 | 45 | -2.38% | 1 | 35.94 | Township |
| Rose Valley township | 19 | 40 | 44 | -2.44% | 1 | 36.44 | Township |
| Albano township | 20 | 37 | 41 | -2.63% | 1 | 36.27 | Township |
| Clear Creek township | 21 | 21 | 22 | 0% | 1 | 36.12 | Township |
| Union township | 22 | 19 | 20 | 0% | 1 | 36.02 | Township |
| Putnam township | 23 | 12 | 12 | 0% | 0.00 | 70.49 | Township |
St. John is the largest city in Stafford County, with a population of 1,137.
Putnam township is the smallest city in Stafford County.
There are 23 cities in Stafford County, Kansas.