16 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Fort Scott | 1 | 7,581 | 7,540 | 0.11% | 1,358 | 5.58 | City |
| Scott township | 2 | 2,278 | 2,266 | -0.09% | 33 | 68.74 | Township |
| Marmaton township | 3 | 721 | 711 | 0.14% | 13 | 55.83 | Township |
| Freedom township | 4 | 595 | 593 | -0.17% | 14 | 43.61 | Township |
| Mill Creek township | 5 | 489 | 488 | -0.2% | 9 | 52.47 | Township |
| Drywood township | 6 | 377 | 374 | -0.26% | 8 | 46.45 | Township |
| Osage township | 7 | 340 | 342 | -0.29% | 7 | 48.15 | Township |
| Bronson | 8 | 308 | 305 | 0.33% | 689 | 0.45 | City |
| Uniontown | 9 | 299 | 293 | 0.34% | 1,417 | 0.21 | City |
| Pawnee township | 10 | 255 | 262 | -1.16% | 5 | 48.47 | Township |
| Timberhill township | 11 | 235 | 233 | 0% | 7 | 35.73 | Township |
| Fulton | 12 | 167 | 166 | 0% | 870 | 0.19 | City |
| Mapleton | 13 | 98 | 97 | 0% | 198 | 0.50 | City |
| Redfield | 14 | 91 | 91 | 0% | 628 | 0.14 | City |
| Devon | 15 | 58 | 51 | 7.41% | 82 | 0.70 | CDP |
| Garland | 16 | 14 | 11 | 7.69% | 65 | 0.21 | CDP |
Fort Scott is the largest city in Bourbon County, with a population of 7,581.
Garland is the smallest city in Bourbon County.
There are 16 cities in Bourbon County, Kansas.