22 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Medicine Lodge township | 1 | 1,914 | 2,028 | -1.34% | 16 | 119.44 | Township |
| Medicine Lodge | 2 | 1,670 | 1,762 | -1.07% | 1,394 | 1.20 | City |
| Kiowa township | 3 | 923 | 977 | -1.28% | 19 | 47.96 | Township |
| Kiowa | 4 | 850 | 897 | -1.05% | 767 | 1.11 | City |
| Sharon township | 5 | 279 | 297 | -1.41% | 4 | 72.72 | Township |
| Hardtner | 6 | 157 | 166 | -1.26% | 571 | 0.28 | City |
| Sharon | 7 | 137 | 147 | -1.44% | 488 | 0.28 | City |
| Hazelton township | 8 | 120 | 125 | -0.83% | 2 | 73.08 | Township |
| Elm Mills township | 9 | 112 | 117 | -0.88% | 2 | 59.67 | Township |
| Valley township | 10 | 111 | 125 | -3.48% | 3 | 36.05 | Township |
| Hazelton | 11 | 80 | 84 | -1.23% | 135 | 0.59 | City |
| Mingona township | 12 | 66 | 69 | -1.49% | 1 | 53.53 | Township |
| Lake City township | 13 | 64 | 67 | -1.54% | 2 | 42 | Township |
| Isabel | 14 | 62 | 66 | -1.59% | 248 | 0.25 | City |
| Moore township | 15 | 52 | 56 | -1.89% | 1 | 99.95 | Township |
| Sun City township | 16 | 46 | 47 | 0% | 1 | 47.88 | Township |
| Sun City | 17 | 34 | 36 | 0% | 233 | 0.15 | City |
| Eagle township | 18 | 26 | 27 | 0% | 0.00 | 111.82 | Township |
| McAdoo township | 19 | 25 | 26 | 0% | 1 | 35.83 | Township |
| Turkey Creek township | 20 | 12 | 12 | 0% | 0.00 | 47.50 | Township |
| Aetna township | 21 | 9 | 9 | 0% | 0.00 | 122.84 | Township |
| Deerhead township | 22 | 9 | 9 | 0% | 0.00 | 63.56 | Township |
Medicine Lodge township is the largest city in Barber County, with a population of 1,914.
Deerhead township is the smallest city in Barber County.
There are 22 cities in Barber County, Kansas.