12 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Maquoketa | 1 | 6,005 | 6,119 | -0.38% | 1,329 | 4.52 | City |
| Bellevue | 2 | 2,295 | 2,357 | -0.52% | 1,665 | 1.38 | City |
| Preston | 3 | 932 | 947 | -0.32% | 1,082 | 0.86 | City |
| Sabula | 4 | 487 | 501 | -0.61% | 986 | 0.49 | City |
| Miles | 5 | 397 | 409 | -0.5% | 314 | 1.26 | City |
| Andrew | 6 | 373 | 377 | -0.27% | 1,504 | 0.25 | City |
| La Motte | 7 | 229 | 238 | -0.87% | 506 | 0.45 | City |
| Springbrook | 8 | 146 | 143 | 0.69% | 241 | 0.60 | City |
| Monmouth | 9 | 129 | 129 | 0% | 227 | 0.57 | City |
| St. Donatus | 10 | 117 | 121 | -0.85% | 313 | 0.37 | City |
| Baldwin | 11 | 99 | 101 | 0% | 261 | 0.38 | City |
| Spragueville | 12 | 89 | 91 | 0% | 120 | 0.74 | City |
Maquoketa is the largest city in Jackson County, with a population of 6,005.
Spragueville is the smallest city in Jackson County.
There are 12 cities in Jackson County, Iowa.