13 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Ames | 1 | 65,152 | 66,487 | -0.41% | 2,310 | 28.20 | City |
| Nevada | 2 | 6,997 | 6,921 | 0.21% | 1,176 | 5.95 | City |
| Huxley | 3 | 4,848 | 4,313 | 2.26% | 1,479 | 3.28 | City |
| Story City | 4 | 3,418 | 3,343 | 0.44% | 1,197 | 2.86 | City |
| Slater | 5 | 1,639 | 1,541 | 1.24% | 857 | 1.91 | City |
| Gilbert | 6 | 1,413 | 1,207 | 2.99% | 1,283 | 1.10 | City |
| Roland | 7 | 1,350 | 1,359 | -0.15% | 1,179 | 1.15 | City |
| Maxwell | 8 | 850 | 859 | -0.23% | 778 | 1.09 | City |
| Colo | 9 | 838 | 847 | -0.24% | 787 | 1.06 | City |
| Cambridge | 10 | 828 | 828 | 0% | 619 | 1.34 | City |
| Collins | 11 | 494 | 498 | -0.2% | 1,012 | 0.49 | City |
| Zearing | 12 | 481 | 535 | -2.24% | 642 | 0.75 | City |
| Kelley | 13 | 310 | 305 | 0.32% | 418 | 0.74 | City |
Ames is the largest city in Story County, with a population of 65,152.
Kelley is the smallest city in Story County.
There are 13 cities in Story County, Iowa.