10 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Stuart | 1 | 1,895 | 1,795 | 1.07% | 673 | 2.82 | City |
| Guthrie Center | 2 | 1,576 | 1,583 | -0.06% | 662 | 2.38 | City |
| Panora | 3 | 1,081 | 1,086 | -0.09% | 597 | 1.81 | City |
| Lake Panorama | 4 | 755 | 805 | -4.67% | 114 | 6.61 | CDP |
| Bayard | 5 | 402 | 406 | -0.25% | 907 | 0.44 | City |
| Casey | 6 | 386 | 383 | 0.26% | 522 | 0.74 | City |
| Menlo | 7 | 343 | 343 | 0% | 692 | 0.50 | City |
| Yale | 8 | 262 | 267 | -0.38% | 907 | 0.29 | City |
| Bagley | 9 | 228 | 232 | -0.44% | 728 | 0.31 | City |
| Jamaica | 10 | 197 | 194 | 0.51% | 530 | 0.37 | City |
Stuart is the largest city in Guthrie County, with a population of 1,895.
Jamaica is the smallest city in Guthrie County.
There are 10 cities in Guthrie County, Iowa.