20 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Dubuque | 1 | 58,359 | 59,654 | -0.44% | 1,869 | 31.23 | City |
| Asbury | 2 | 6,037 | 5,961 | 0.25% | 1,900 | 3.18 | City |
| Dyersville | 3 | 4,591 | 4,505 | 0.37% | 660 | 6.96 | City |
| Cascade | 4 | 2,381 | 2,390 | -0.08% | 1,239 | 1.92 | City |
| Peosta | 5 | 2,250 | 1,930 | 2.93% | 871 | 2.58 | City |
| Epworth | 6 | 2,026 | 2,020 | 0.05% | 1,141 | 1.78 | City |
| Farley | 7 | 1,692 | 1,767 | -0.88% | 1,044 | 1.62 | City |
| New Vienna | 8 | 378 | 383 | -0.26% | 811 | 0.47 | City |
| Worthington | 9 | 368 | 378 | -0.54% | 814 | 0.45 | City |
| Holy Cross | 10 | 340 | 360 | -1.16% | 1,037 | 0.33 | City |
| Luxemburg | 11 | 235 | 242 | -0.42% | 498 | 0.47 | City |
| Rickardsville | 12 | 201 | 206 | -0.5% | 218 | 0.92 | City |
| Sherrill | 13 | 171 | 183 | -1.16% | 1,295 | 0.13 | City |
| Centralia | 14 | 117 | 116 | 0% | 188 | 0.62 | City |
| Bernard | 15 | 116 | 115 | 0% | 1,247 | 0.09 | City |
| Sageville | 16 | 91 | 96 | -1.09% | 108 | 0.84 | City |
| Zwingle | 17 | 86 | 86 | 0% | 528 | 0.16 | City |
| Graf | 18 | 75 | 74 | 0% | 564 | 0.13 | City |
| Bankston | 19 | 22 | 24 | 0% | 81 | 0.27 | City |
| Durango | 20 | 20 | 22 | 0% | 263 | 0.08 | City |
Dubuque is the largest city in Dubuque County, with a population of 58,359.
Durango is the smallest city in Dubuque County.
There are 20 cities in Dubuque County, Iowa.