37 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Hanover township | 1 | 95,022 | 99,705 | -0.53% | 2,846 | 33.38 | Township |
| Warren township | 2 | 64,351 | 65,760 | -0.42% | 1,790 | 35.95 | Township |
| Dunleith township | 3 | 3,512 | 3,548 | -0.14% | 368 | 9.53 | Township |
| Galena | 4 | 3,205 | 3,317 | -0.68% | 694 | 4.62 | City |
| West Galena township | 5 | 3,144 | 3,246 | -0.6% | 327 | 9.61 | Township |
| Stockton township | 6 | 2,211 | 2,281 | -0.58% | 60 | 37.09 | Township |
| The Galena Territory | 7 | 1,743 | 1,424 | 2.71% | 158 | 11.01 | CDP |
| Stockton | 8 | 1,669 | 1,733 | -0.77% | 889 | 1.88 | Village |
| Guilford township | 9 | 1,515 | 1,536 | -0.26% | 41 | 37.11 | Township |
| East Dubuque | 10 | 1,473 | 1,503 | -0.41% | 512 | 2.88 | City |
| Warren township | 11 | 1,434 | 1,489 | -0.69% | 73 | 19.71 | Township |
| Warren | 12 | 1,276 | 1,330 | -0.85% | 1,235 | 1.03 | Village |
| East Galena township | 13 | 1,230 | 1,256 | -0.4% | 53 | 23.33 | Township |
| Hanover township | 14 | 1,157 | 1,191 | -0.52% | 22 | 52.03 | Township |
| Menominee township | 15 | 1,031 | 1,046 | -0.19% | 37 | 27.94 | Township |
| Elizabeth township | 16 | 979 | 1,000 | -0.41% | 27 | 36.88 | Township |
| Thompson township | 17 | 830 | 841 | -0.24% | 23 | 36.21 | Township |
| Hanover | 18 | 827 | 862 | -0.84% | 737 | 1.12 | Village |
| Apple Canyon Lake | 19 | 740 | 639 | 1.65% | 199 | 3.72 | CDP |
| Elizabeth | 20 | 688 | 705 | -0.43% | 746 | 0.92 | Village |
| Scales Mound township | 21 | 635 | 658 | -0.94% | 34 | 18.54 | Township |
| Woodbine township | 22 | 521 | 528 | -0.19% | 14 | 36.73 | Township |
| Rawlins township | 23 | 509 | 519 | -0.39% | 43 | 11.72 | Township |
| Apple River township | 24 | 435 | 447 | -0.68% | 23 | 18.52 | Township |
| Scales Mound | 25 | 421 | 433 | -0.47% | 670 | 0.63 | Village |
| Vinegar Hill township | 26 | 396 | 404 | -0.5% | 29 | 13.80 | Township |
| Rush township | 27 | 380 | 382 | 0% | 10 | 36.77 | Township |
| Rice township | 28 | 345 | 350 | -0.29% | 14 | 24.40 | Township |
| Nora township | 29 | 333 | 337 | -0.3% | 13 | 25.35 | Township |
| Apple River | 30 | 332 | 339 | -0.3% | 471 | 0.70 | Village |
| Derinda township | 31 | 279 | 284 | -0.36% | 8 | 36.96 | Township |
| Wards Grove township | 32 | 222 | 222 | 0.45% | 12 | 17.89 | Township |
| Menominee | 33 | 210 | 212 | 0% | 110 | 1.91 | Village |
| Pleasant Valley township | 34 | 206 | 210 | -0.48% | 6 | 36.58 | Township |
| Berreman township | 35 | 143 | 148 | -0.69% | 8 | 17.81 | Township |
| Council Hill township | 36 | 131 | 134 | -0.76% | 8 | 16.43 | Township |
| Nora | 37 | 109 | 109 | 0% | 131 | 0.83 | Village |
Hanover township is the largest city in Jo Daviess County, with a population of 95,022.
Nora is the smallest city in Jo Daviess County.
There are 37 cities in Jo Daviess County, Illinois.