10 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Darlington | 1 | 2,546 | 2,453 | 0.75% | 1,543 | 1.65 | City |
| Shullsburg | 2 | 1,205 | 1,172 | 0.58% | 905 | 1.33 | City |
| Belmont | 3 | 1,014 | 993 | 0.4% | 1,186 | 0.85 | Village |
| Benton | 4 | 984 | 943 | 0.82% | 1,167 | 0.84 | Village |
| Blanchardville | 5 | 827 | 814 | 0.36% | 1,523 | 0.54 | Village |
| Argyle | 6 | 801 | 785 | 0.38% | 1,269 | 0.63 | Village |
| South Wayne | 7 | 446 | 445 | 0% | 537 | 0.83 | Village |
| Gratiot | 8 | 221 | 223 | 0% | 403 | 0.55 | Village |
| Woodford | 9 | 124 | 45 | 6.9% | 515 | 0.24 | CDP |
| Wiota | 10 | 104 | 128 | 7.22% | 52 | 2 | CDP |
Darlington is the largest city in Lafayette County, with a population of 2,546.
Wiota is the smallest city in Lafayette County.
There are 10 cities in Lafayette County, Wisconsin.