11 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Mauston | 1 | 4,060 | 4,349 | -1.41% | 908 | 4.47 | City |
| New Lisbon | 2 | 2,509 | 2,578 | -0.55% | 918 | 2.73 | City |
| Elroy | 3 | 1,306 | 1,356 | -0.76% | 650 | 2.01 | City |
| Necedah | 4 | 900 | 932 | -0.66% | 326 | 2.76 | Village |
| Wonewoc | 5 | 722 | 761 | -1.1% | 646 | 1.12 | Village |
| Camp Douglas | 6 | 629 | 644 | -0.47% | 626 | 1 | Village |
| Lyndon Station | 7 | 477 | 494 | -0.63% | 226 | 2.11 | Village |
| Ironton | 8 | 262 | 274 | -0.76% | 787 | 0.33 | Village |
| Union Center | 9 | 213 | 222 | -0.93% | 258 | 0.82 | Village |
| Hustler | 10 | 160 | 167 | -0.62% | 229 | 0.70 | Village |
| Yuba | 11 | 50 | 52 | 0% | 164 | 0.30 | Village |
Mauston is the largest city in Juneau County, with a population of 4,060.
Yuba is the smallest city in Juneau County.
There are 11 cities in Juneau County, Wisconsin.