11 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Viroqua | 1 | 4,393 | 4,502 | -0.5% | 988 | 4.45 | City |
| Westby | 2 | 2,390 | 2,330 | 0.5% | 887 | 2.69 | City |
| Hillsboro | 3 | 1,436 | 1,396 | 0.56% | 1,018 | 1.41 | City |
| Stoddard | 4 | 872 | 834 | 0.93% | 1,369 | 0.64 | Village |
| Coon Valley | 5 | 765 | 757 | 0.26% | 687 | 1.11 | Village |
| La Farge | 6 | 716 | 721 | -0.14% | 710 | 1.01 | Village |
| Ontario | 7 | 537 | 536 | 0% | 535 | 1 | Village |
| Readstown | 8 | 375 | 372 | 0.27% | 219 | 1.71 | Village |
| De Soto | 9 | 323 | 308 | 0.94% | 278 | 1.16 | Village |
| Chaseburg | 10 | 243 | 242 | 0% | 288 | 0.84 | Village |
| Genoa | 11 | 231 | 236 | -0.43% | 647 | 0.36 | Village |
Viroqua is the largest city in Vernon County, with a population of 4,393.
Genoa is the smallest city in Vernon County.
There are 11 cities in Vernon County, Wisconsin.