8 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Mondovi | 1 | 2,852 | 2,842 | 0.07% | 732 | 3.90 | City |
| Buffalo City | 2 | 1,017 | 1,014 | 0.1% | 537 | 1.89 | City |
| Fountain City | 3 | 761 | 803 | -1.04% | 174 | 4.38 | City |
| Alma | 4 | 719 | 714 | 0.14% | 135 | 5.31 | City |
| Cochrane | 5 | 415 | 424 | -0.48% | 536 | 0.77 | Village |
| Nelson | 6 | 318 | 320 | 0% | 235 | 1.35 | Village |
| Gilmanton | 7 | 178 | 122 | 7.23% | 53 | 3.35 | CDP |
| Waumandee | 8 | 89 | 73 | 7.23% | 511 | 0.17 | CDP |
Mondovi is the largest city in Buffalo County, with a population of 2,852.
Waumandee is the smallest city in Buffalo County.
There are 8 cities in Buffalo County, Wisconsin.