12 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Arcadia | 1 | 3,834 | 3,738 | 0.5% | 1,172 | 3.27 | City |
| Trempealeau | 2 | 1,901 | 1,848 | 0.58% | 959 | 1.98 | Village |
| Osseo | 3 | 1,771 | 1,810 | -0.45% | 861 | 2.06 | City |
| Galesville | 4 | 1,696 | 1,660 | 0.41% | 1,304 | 1.30 | City |
| Whitehall | 5 | 1,589 | 1,656 | -0.81% | 418 | 3.81 | City |
| Independence | 6 | 1,462 | 1,487 | -0.34% | 351 | 4.16 | City |
| Blair | 7 | 1,235 | 1,322 | -1.36% | 287 | 4.30 | City |
| Strum | 8 | 1,042 | 1,079 | -0.67% | 849 | 1.23 | Village |
| Eleva | 9 | 682 | 684 | 0% | 1,218 | 0.56 | Village |
| Ettrick | 10 | 516 | 528 | -0.39% | 698 | 0.74 | Village |
| Pigeon Falls | 11 | 366 | 381 | -0.81% | 843 | 0.43 | Village |
| Dodge | 12 | 252 | 141 | 3.28% | 541 | 0.47 | CDP |
Arcadia is the largest city in Trempealeau County, with a population of 3,834.
Dodge is the smallest city in Trempealeau County.
There are 12 cities in Trempealeau County, Wisconsin.