4 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Wheeling | 1 | 25,682 | 26,996 | -1.01% | 1,863 | 13.79 | City |
| West Liberty | 2 | 1,431 | 1,456 | -0.35% | 1,310 | 1.09 | Town |
| Triadelphia | 3 | 637 | 671 | -1.09% | 947 | 0.67 | Town |
| Valley Grove | 4 | 258 | 275 | -1.15% | 472 | 0.55 | Village |
Wheeling is the largest city in Ohio County, with a population of 25,682.
Valley Grove is the smallest city in Ohio County.
There are 4 cities in Ohio County, West Virginia.