5 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Martinsburg | 1 | 19,042 | 18,774 | 0.28% | 2,872 | 6.63 | City |
| Inwood | 2 | 2,800 | 2,700 | -0.36% | 981 | 2.85 | CDP |
| Falling Waters | 3 | 2,468 | 1,991 | 4.49% | 1,963 | 1.26 | CDP |
| Middleway | 4 | 440 | 396 | 0% | 393 | 1.12 | CDP |
| Hedgesville | 5 | 299 | 304 | -0.33% | 2,282 | 0.13 | Town |
Martinsburg is the largest city in Berkeley County, with a population of 19,042.
Hedgesville is the smallest city in Berkeley County.
There are 5 cities in Berkeley County, West Virginia.