9 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Madison | 1 | 2,634 | 2,904 | -2.01% | 376 | 7.01 | City |
| Danville | 2 | 618 | 673 | -1.75% | 578 | 1.07 | Town |
| Racine | 3 | 507 | 476 | 3.47% | 1,058 | 0.48 | CDP |
| Whitesville | 4 | 321 | 361 | -2.43% | 1,046 | 0.31 | Town |
| Greenview | 5 | 204 | 281 | -5.56% | 207 | 0.99 | CDP |
| Sylvester | 6 | 146 | 166 | -2.67% | 579 | 0.25 | Town |
| Comfort | 7 | 143 | 225 | -7.14% | 142 | 1 | CDP |
| Van | 8 | 114 | 138 | -5% | 191 | 0.60 | CDP |
| Twilight | 9 | 104 | 42 | 7.22% | 208 | 0.50 | CDP |
Madison is the largest city in Boone County, with a population of 2,634.
Twilight is the smallest city in Boone County.
There are 9 cities in Boone County, West Virginia.