8 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Tupelo | 1 | 37,511 | 37,921 | -0.22% | 583 | 64.38 | City |
| Saltillo | 2 | 4,956 | 4,953 | 0.02% | 520 | 9.54 | City |
| Baldwyn | 3 | 3,064 | 3,068 | -0.03% | 264 | 11.59 | City |
| Verona | 4 | 2,692 | 2,792 | -0.74% | 733 | 3.67 | City |
| Guntown | 5 | 2,439 | 2,408 | 0.25% | 523 | 4.66 | Town |
| Mooreville | 6 | 2,136 | 2,138 | 4.86% | 764 | 2.79 | CDP |
| Shannon | 7 | 1,470 | 1,494 | -0.34% | 363 | 4.05 | Town |
| Plantersville | 8 | 828 | 865 | -0.84% | 396 | 2.09 | Town |
Tupelo is the largest city in Lee County, with a population of 37,511.
Plantersville is the smallest city in Lee County.
There are 8 cities in Lee County, Mississippi.