7 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Madison | 1 | 63,223 | 57,300 | 1.91% | 2,069 | 30.56 | City |
| Athens | 2 | 34,368 | 25,707 | 5.31% | 846 | 40.60 | City |
| Triana | 3 | 5,427 | 2,999 | 9.84% | 1,938 | 2.80 | Town |
| Ardmore | 4 | 1,477 | 1,336 | 1.93% | 723 | 2.04 | Town |
| Elkmont | 5 | 450 | 414 | 1.58% | 274 | 1.64 | Town |
| Lester | 6 | 123 | 112 | 1.65% | 64 | 1.91 | Town |
| Mooresville | 7 | 49 | 46 | 2.08% | 196 | 0.25 | Town |
Madison is the largest city in Limestone County, with a population of 63,223.
Mooresville is the smallest city in Limestone County.
There are 7 cities in Limestone County, Alabama.