24 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| La Crescent | 1 | 5,264 | 5,338 | -0.28% | 1,565 | 3.36 | City |
| Caledonia | 2 | 2,756 | 2,851 | -0.68% | 976 | 2.82 | City |
| Spring Grove | 3 | 1,195 | 1,254 | -0.99% | 1,202 | 0.99 | City |
| La Crescent township | 4 | 1,029 | 1,057 | -0.68% | 51 | 20.17 | Township |
| Houston | 5 | 972 | 999 | -0.51% | 790 | 1.23 | City |
| Caledonia township | 6 | 617 | 635 | -0.8% | 18 | 33.77 | Township |
| Money Creek township | 7 | 569 | 577 | -0.35% | 16 | 35.56 | Township |
| Mound Prairie township | 8 | 554 | 570 | -0.72% | 15 | 36.26 | Township |
| Brownsville | 9 | 543 | 567 | -0.91% | 294 | 1.85 | City |
| Hokah | 10 | 541 | 555 | -0.55% | 774 | 0.70 | City |
| Hokah township | 11 | 433 | 444 | -0.69% | 19 | 22.78 | Township |
| Wilmington township | 12 | 417 | 427 | -0.48% | 12 | 35.87 | Township |
| Brownsville township | 13 | 403 | 414 | -0.74% | 14 | 28.19 | Township |
| Houston township | 14 | 384 | 396 | -0.78% | 12 | 32.93 | Township |
| Mayville township | 15 | 348 | 363 | -1.42% | 11 | 30.52 | Township |
| Spring Grove township | 16 | 332 | 341 | -0.6% | 10 | 34.84 | Township |
| Dresbach | 17 | 324 | 256 | 7.28% | 343 | 0.94 | CDP |
| Yucatan township | 18 | 320 | 328 | -0.62% | 7 | 42.73 | Township |
| Union township | 19 | 314 | 322 | -0.63% | 12 | 26.20 | Township |
| Sheldon township | 20 | 295 | 300 | -0.34% | 10 | 29.70 | Township |
| Black Hammer township | 21 | 269 | 274 | -0.37% | 8 | 35.63 | Township |
| Eitzen | 22 | 268 | 280 | -0.74% | 439 | 0.61 | City |
| Crooked Creek township | 23 | 246 | 250 | -0.4% | 8 | 31.24 | Township |
| Jefferson township | 24 | 129 | 132 | -0.77% | 4 | 29.25 | Township |
La Crescent is the largest city in Houston County, with a population of 5,264.
Jefferson township is the smallest city in Houston County.
There are 24 cities in Houston County, Minnesota.