23 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Jackson | 1 | 3,240 | 3,320 | -0.49% | 624 | 5.19 | City |
| Lakefield | 2 | 1,707 | 1,732 | -0.29% | 1,276 | 1.34 | City |
| Jackson township | 3 | 1,633 | 1,612 | -0.18% | 313 | 5.22 | Township |
| Heron Lake | 4 | 583 | 602 | -0.68% | 469 | 1.24 | City |
| Heron Lake township | 5 | 316 | 309 | 0.96% | 8 | 38.61 | Township |
| Christiania township | 6 | 272 | 268 | 0.74% | 8 | 35.62 | Township |
| Des Moines township | 7 | 235 | 236 | 0% | 8 | 30.56 | Township |
| Delafield township | 8 | 232 | 230 | 0.43% | 7 | 34.72 | Township |
| Petersburg township | 9 | 223 | 224 | 0% | 6 | 36.14 | Township |
| Middletown township | 10 | 218 | 216 | 0.46% | 6 | 35.67 | Township |
| Belmont township | 11 | 217 | 217 | 0% | 6 | 35.99 | Township |
| Hunter township | 12 | 215 | 213 | 0.47% | 6 | 35.71 | Township |
| Ewington township | 13 | 212 | 210 | 0.47% | 6 | 35.73 | Township |
| Wisconsin township | 14 | 211 | 211 | 0% | 6 | 35.36 | Township |
| Rost township | 15 | 208 | 208 | 0% | 6 | 36.10 | Township |
| Okabena | 16 | 207 | 207 | 0% | 612 | 0.34 | City |
| Sioux Valley township | 17 | 197 | 197 | 0% | 5 | 35.93 | Township |
| Enterprise township | 18 | 185 | 181 | 1.09% | 5 | 35.93 | Township |
| Alba township | 19 | 169 | 165 | 1.2% | 5 | 35.25 | Township |
| Weimer township | 20 | 159 | 156 | 0.63% | 5 | 31.16 | Township |
| La Crosse township | 21 | 149 | 145 | 1.36% | 4 | 34.90 | Township |
| West Heron Lake township | 22 | 128 | 127 | 0% | 5 | 27.12 | Township |
| Alpha | 23 | 94 | 99 | -1.05% | 439 | 0.21 | City |
Jackson is the largest city in Jackson County, with a population of 3,240.
Alpha is the smallest city in Jackson County.
There are 23 cities in Jackson County, Minnesota.