Note: As defined by the US Census Bureau, a city's official boundaries often extend miles beyond the city itself and may even cross county lines. As such, several thousand US cities officially exist in two or more counties (and therefore appear on multiple counties' lists of cities.)
City | Rank | 2024 Pop. | 2020 Pop. | Change | Density (/mi²) | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Caro | 1 | 4,255 | 4,322 | -0.4% | 1,445 | 2.9 | City |
| Millington township | 2 | 4,182 | 4,240 | -0.21% | 118 | 35.5 | Township |
| Vassar township | 3 | 3,890 | 3,883 | 0.15% | 111 | 35.0 | Township |
| Elkland township | 4 | 3,487 | 3,529 | -0.17% | 100 | 35.0 | Township |
| Arbela township | 5 | 2,768 | 2,808 | -0.32% | 83 | 33.5 | Township |
| Denmark township | 6 | 2,712 | 2,747 | -0.18% | 77 | 35.3 | Township |
| Vassar | 7 | 2,687 | 2,729 | -0.41% | 1,251 | 2.1 | City |
| Cass City | 8 | 2,478 | 2,513 | -0.36% | 1,404 | 1.8 | Village |
| Indianfields township | 9 | 2,460 | 2,475 | 0.04% | 78 | 31.4 | Township |
| Watertown township | 10 | 2,074 | 2,093 | -0.05% | 64 | 32.5 | Township |
| Almer township | 11 | 1,976 | 1,983 | 0.05% | 58 | 33.8 | Township |
| Tuscola township | 12 | 1,939 | 1,975 | -0.41% | 59 | 32.8 | Township |
| Dayton township | 13 | 1,904 | 1,922 | -0.1% | 53 | 35.6 | Township |
| Juniata township | 14 | 1,553 | 1,558 | 0.06% | 44 | 35.2 | Township |
| Fairgrove township | 15 | 1,531 | 1,553 | -0.26% | 43 | 35.3 | Township |
| Koylton township | 16 | 1,477 | 1,482 | 0.07% | 42 | 35.1 | Township |
| Kingston township | 17 | 1,459 | 1,477 | -0.21% | 41 | 35.2 | Township |
| Novesta township | 18 | 1,427 | 1,442 | -0.21% | 40 | 35.3 | Township |
| Akron township | 19 | 1,356 | 1,377 | -0.22% | 26 | 52.8 | Township |
| Ellington township | 20 | 1,319 | 1,328 | 0% | 37 | 35.4 | Township |
| Reese | 21 | 1,263 | 1,282 | -0.39% | 935 | 1.4 | Village |
| Columbia township | 22 | 1,206 | 1,229 | -0.41% | 34 | 36.0 | Township |
| Elmwood township | 23 | 1,047 | 1,062 | -0.29% | 30 | 35.4 | Township |
| Millington | 24 | 1,014 | 1,034 | -0.49% | 744 | 1.4 | Village |
| Mayville | 25 | 903 | 921 | -0.55% | 838 | 1.1 | Village |
| Fairgrove | 26 | 523 | 534 | -0.57% | 467 | 1.1 | Village |
| Unionville | 27 | 482 | 492 | -0.62% | 513 | 0.9 | Village |
| Kingston | 28 | 393 | 401 | -0.51% | 386 | 1.0 | Village |
| Akron | 29 | 367 | 374 | -0.54% | 390 | 0.9 | Village |
| Gagetown | 30 | 316 | 323 | -0.63% | 322 | 1.0 | Village |
Note: City markers shown outside the county boundary mean that a part of the city extends into this county, but is primarily located in an adjacent county.
Caro is the largest city in Tuscola County.
Gagetown is the smallest city in Tuscola County.
There are 30 cities in Tuscola County, Michigan.