33 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Caro | 1 | 4,238 | 4,322 | -0.4% | 1,440 | 2.94 | City |
| Millington township | 2 | 4,173 | 4,240 | -0.22% | 117 | 35.52 | Township |
| Vassar township | 3 | 3,896 | 3,883 | 0.15% | 111 | 35.04 | Township |
| Elkland township | 4 | 3,481 | 3,529 | -0.17% | 99 | 35 | Township |
| Arbela township | 5 | 2,759 | 2,808 | -0.33% | 82 | 33.46 | Township |
| Denmark township | 6 | 2,707 | 2,747 | -0.18% | 77 | 35.27 | Township |
| Vassar | 7 | 2,677 | 2,729 | -0.37% | 1,246 | 2.15 | City |
| Cass City | 8 | 2,469 | 2,513 | -0.36% | 1,399 | 1.76 | Village |
| Indianfields township | 9 | 2,461 | 2,475 | 0.04% | 78 | 31.39 | Township |
| Watertown township | 10 | 2,073 | 2,093 | -0.05% | 64 | 32.53 | Township |
| Almer township | 11 | 1,977 | 1,983 | 0.05% | 58 | 33.84 | Township |
| Tuscola township | 12 | 1,931 | 1,975 | -0.41% | 59 | 32.78 | Township |
| Dayton township | 13 | 1,902 | 1,922 | -0.11% | 53 | 35.59 | Township |
| Juniata township | 14 | 1,554 | 1,558 | 0.06% | 44 | 35.18 | Township |
| Fairgrove township | 15 | 1,527 | 1,553 | -0.26% | 43 | 35.26 | Township |
| Koylton township | 16 | 1,478 | 1,482 | 0.07% | 42 | 35.06 | Township |
| Kingston township | 17 | 1,456 | 1,477 | -0.21% | 41 | 35.16 | Township |
| Novesta township | 18 | 1,424 | 1,442 | -0.21% | 40 | 35.30 | Township |
| Akron township | 19 | 1,353 | 1,377 | -0.22% | 26 | 52.76 | Township |
| Ellington township | 20 | 1,319 | 1,328 | 0% | 37 | 35.44 | Township |
| Reese | 21 | 1,258 | 1,282 | -0.4% | 931 | 1.35 | Village |
| Columbia township | 22 | 1,201 | 1,229 | -0.41% | 33 | 35.98 | Township |
| Fostoria | 23 | 1,105 | 752 | 2.5% | 276 | 4.01 | CDP |
| Elmwood township | 24 | 1,044 | 1,062 | -0.29% | 29 | 35.40 | Township |
| Millington | 25 | 1,009 | 1,034 | -0.49% | 741 | 1.36 | Village |
| Mayville | 26 | 899 | 921 | -0.44% | 835 | 1.08 | Village |
| Gilford township | 27 | 722 | 739 | -0.41% | 21 | 34.82 | Township |
| Wisner township | 28 | 593 | 604 | -0.34% | 31 | 19.31 | Township |
| Fairgrove | 29 | 520 | 534 | -0.57% | 464 | 1.12 | Village |
| Unionville | 30 | 480 | 492 | -0.41% | 511 | 0.94 | Village |
| Kingston | 31 | 391 | 401 | -0.51% | 384 | 1.02 | Village |
| Akron | 32 | 365 | 374 | -0.54% | 387 | 0.94 | Village |
| Gagetown | 33 | 314 | 323 | -0.63% | 320 | 0.98 | Village |
Caro is the largest city in Tuscola County, with a population of 4,238.
Gagetown is the smallest city in Tuscola County.
There are 33 cities in Tuscola County, Michigan.