24 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Chamberlain | 1 | 2,502 | 2,474 | 0.24% | 559 | 4.48 | City |
| Kimball | 2 | 588 | 570 | 0.68% | 171 | 3.44 | City |
| Wilbur township | 3 | 250 | 250 | 0% | 7 | 35.81 | Township |
| Pukwana | 4 | 241 | 233 | 0.84% | 314 | 0.77 | Town |
| Chamberlain township | 5 | 238 | 233 | 0.42% | 12 | 19.98 | Township |
| Torrey Lake township | 6 | 221 | 219 | -0.45% | 6 | 35.63 | Township |
| Brule township | 7 | 169 | 163 | 0.6% | 4 | 41.90 | Township |
| Pukwana township | 8 | 153 | 148 | 0.66% | 4 | 35.41 | Township |
| Ola township | 9 | 100 | 98 | 0% | 3 | 35.92 | Township |
| West Point township | 10 | 100 | 101 | -0.99% | 2 | 47.33 | Township |
| Cleveland township | 11 | 88 | 88 | -1.12% | 2 | 36.87 | Township |
| Red Lake township | 12 | 87 | 79 | 2.35% | 3 | 30.16 | Township |
| Union township | 13 | 73 | 73 | -1.35% | 2 | 35.52 | Township |
| Waldro township | 14 | 72 | 72 | -1.37% | 2 | 36.77 | Township |
| Richland township | 15 | 53 | 54 | -1.85% | 1 | 35.59 | Township |
| Kimball township | 16 | 50 | 44 | 4.17% | 2 | 32.08 | Township |
| Smith township | 17 | 45 | 46 | -2.17% | 1 | 35.86 | Township |
| America township | 18 | 44 | 45 | -2.22% | 1 | 42.27 | Township |
| Pleasant Grove township | 19 | 38 | 38 | 0% | 1 | 35.38 | Township |
| Eagle township | 20 | 37 | 38 | -2.63% | 1 | 35.47 | Township |
| Plainfield township | 21 | 36 | 36 | 0% | 1 | 35.47 | Township |
| Highland township | 22 | 34 | 31 | 3.03% | 1 | 35.81 | Township |
| Lyon township | 23 | 27 | 27 | 0% | 1 | 35.48 | Township |
| Plummer township | 24 | 19 | 20 | -5% | 1 | 35.54 | Township |
Chamberlain is the largest city in Brule County, with a population of 2,502.
Plummer township is the smallest city in Brule County.
There are 24 cities in Brule County, South Dakota.