67 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Bangor city | 1 | 31,662 | 31,720 | 0.05% | 924 | 34.26 | City |
| Bangor | 2 | 31,566 | 31,720 | -0.1% | 921 | 34.26 | City |
| Orono town | 3 | 12,259 | 11,167 | 0.02% | 674 | 18.19 | Town |
| Brewer | 4 | 9,632 | 9,667 | -0.07% | 632 | 15.24 | City |
| Brewer city | 5 | 9,598 | 9,667 | -0.25% | 630 | 15.24 | City |
| Hampden town | 6 | 8,145 | 7,735 | 1.05% | 215 | 37.94 | Town |
| Old Town | 7 | 7,434 | 7,426 | 0.03% | 192 | 38.81 | City |
| Old Town city | 8 | 7,389 | 7,426 | -0.28% | 190 | 38.81 | City |
| Hermon town | 9 | 6,996 | 6,481 | 1.23% | 195 | 35.87 | Town |
| Lincoln town | 10 | 4,876 | 4,847 | 0.1% | 72 | 67.83 | Town |
| Glenburn town | 11 | 4,801 | 4,653 | 0.33% | 177 | 27.16 | Town |
| Millinocket town | 12 | 4,084 | 4,107 | -0.22% | 257 | 15.90 | Town |
| Orrington town | 13 | 3,922 | 3,809 | 0.62% | 157 | 24.99 | Town |
| Dexter town | 14 | 3,816 | 3,801 | 0.05% | 109 | 35.14 | Town |
| Holden town | 15 | 3,451 | 3,281 | 0.76% | 110 | 31.32 | Town |
| Levant town | 16 | 3,423 | 2,939 | 3.73% | 114 | 30.06 | Town |
| Newport town | 17 | 3,158 | 3,129 | 0.06% | 107 | 29.51 | Town |
| Milford town | 18 | 3,115 | 3,069 | 0.1% | 68 | 45.63 | Town |
| Corinth town | 19 | 3,099 | 2,901 | 1.51% | 77 | 40.27 | Town |
| Carmel town | 20 | 3,028 | 2,870 | 0.93% | 83 | 36.65 | Town |
| Milford | 21 | 2,279 | 2,356 | 0.13% | 682 | 3.34 | CDP |
| Eddington town | 22 | 2,257 | 2,187 | 0.4% | 90 | 24.99 | Town |
| Corinna town | 23 | 2,250 | 2,217 | 0.22% | 58 | 38.71 | Town |
| Veazie town | 24 | 1,814 | 1,815 | -0.22% | 600 | 3.02 | Town |
| Newburgh town | 25 | 1,734 | 1,599 | 1.64% | 56 | 31.01 | Town |
| Charleston town | 26 | 1,639 | 1,552 | 0.18% | 40 | 40.55 | Town |
| Bradley town | 27 | 1,591 | 1,534 | 0.7% | 32 | 49.52 | Town |
| East Millinocket town | 28 | 1,562 | 1,567 | -0.19% | 220 | 7.09 | Town |
| Greenbush town | 29 | 1,474 | 1,446 | 0.07% | 34 | 43.80 | Town |
| Hudson town | 30 | 1,462 | 1,418 | 0.83% | 39 | 37.64 | Town |
| Enfield town | 31 | 1,439 | 1,432 | -0.07% | 52 | 27.73 | Town |
| Kenduskeag town | 32 | 1,427 | 1,344 | 0.78% | 85 | 16.75 | Town |
| Plymouth town | 33 | 1,324 | 1,326 | -0.23% | 45 | 29.73 | Town |
| Stetson town | 34 | 1,241 | 1,189 | 0.98% | 35 | 35.01 | Town |
| Etna town | 35 | 1,236 | 1,228 | 0% | 50 | 24.69 | Town |
| Bradford town | 36 | 1,225 | 1,181 | 0.74% | 30 | 41.18 | Town |
| Howland town | 37 | 1,217 | 1,100 | 2.44% | 35 | 34.94 | Town |
| Dixmont town | 38 | 1,205 | 1,210 | -0.25% | 33 | 36.29 | Town |
| Medway town | 39 | 1,188 | 1,185 | -0.08% | 29 | 41 | Town |
| Garland town | 40 | 1,028 | 1,021 | 0% | 27 | 37.67 | Town |
| Exeter town | 41 | 998 | 965 | 0.6% | 26 | 38.56 | Town |
| Lee town | 42 | 907 | 917 | -0.44% | 23 | 38.66 | Town |
| Patten town | 43 | 897 | 880 | 0.34% | 23 | 38.19 | Town |
| Clifton town | 44 | 884 | 844 | 1.03% | 26 | 34.53 | Town |
| Alton town | 45 | 835 | 828 | 0% | 20 | 42.32 | Town |
| Corinna | 46 | 695 | 702 | 7.09% | 326 | 2.13 | CDP |
| Lagrange town | 47 | 645 | 636 | 0.16% | 13 | 49.47 | Town |
| Mattawamkeag town | 48 | 600 | 594 | 0% | 16 | 37.73 | Town |
| Chester town | 49 | 554 | 547 | 0.18% | 12 | 45.83 | Town |
| Mattawamkeag | 50 | 539 | 583 | 7.16% | 190 | 2.84 | CDP |
| Patten | 51 | 475 | 437 | 7.22% | 239 | 1.99 | CDP |
| Burlington town | 52 | 451 | 382 | 2.97% | 8 | 54.01 | Town |
| Winn town | 53 | 403 | 401 | -0.25% | 9 | 43.70 | Town |
| Springfield town | 54 | 398 | 297 | 6.99% | 10 | 38.48 | Town |
| Lowell town | 55 | 390 | 367 | 1.04% | 10 | 38.28 | Town |
| Stacyville town | 56 | 372 | 374 | -0.27% | 9 | 39.53 | Town |
| Penobscot Indian Island Reservation | 57 | 371 | 369 | -0.27% | 49 | 7.56 | Township |
| Passadumkeag town | 58 | 360 | 354 | 0.28% | 16 | 22.92 | Town |
| Woodville town | 59 | 203 | 205 | -0.49% | 5 | 42.73 | Town |
| Mount Chase town | 60 | 188 | 187 | 0% | 5 | 36.65 | Town |
| Carroll plantation | 61 | 140 | 137 | 0.72% | 3 | 43.95 | Plantation |
| Edinburg town | 62 | 135 | 133 | 0% | 4 | 35.01 | Town |
| Lakeville town | 63 | 104 | 103 | 0% | 2 | 58.35 | Town |
| Maxfield town | 64 | 79 | 80 | -1.25% | 4 | 18.93 | Town |
| Webster plantation | 65 | 68 | 65 | 1.49% | 2 | 36.65 | Plantation |
| Seboeis plantation | 66 | 41 | 38 | 2.5% | 1 | 40.01 | Plantation |
| Drew plantation | 67 | 26 | 26 | 0% | 1 | 38.03 | Plantation |
Bangor city is the largest city in Penobscot County, with a population of 31,662.
Drew plantation is the smallest city in Penobscot County.
There are 67 cities in Penobscot County, Maine.