City | Rank | County | 2024 Pop. |
|---|---|---|---|
| Gallatin | 1 | Sumner County | 52,197 |
| White House | 2 | Robertson County | 16,093 |
| Portland | 3 | Sumner County | 13,701 |
| Hartsville/Trousdale County | 4 | Macon County | 12,483 |
| Greenbrier | 5 | Robertson County | 6,949 |
| Millersville | 6 | Davidson County | 6,294 |
| Lafayette | 7 | Macon County | 5,920 |
| Shackle Island | 8 | Davidson County | 4,630 |
| Westmoreland | 9 | Sumner County | 2,803 |
| Ridgetop | 10 | Robertson County | 2,143 |
| Cross Plains | 11 | Robertson County | 1,929 |
| Red Boiling Springs | 12 | Macon County | 1,217 |
| Orlinda | 13 | Robertson County | 1,008 |
| Castalian Springs | 14 | Sumner County | 631 |
| Walnut Grove | 15 | Robertson County | 626 |
| Cottontown | 16 | Sumner County | 341 |
| Fairfield | 17 | Macon County | 288 |
| Bethpage | 18 | Sumner County | 274 |
| New Deal | 19 | Robertson County | 246 |
| Mitchellville | 20 | Sumner County | 157 |
| Oak Grove | 21 | Macon County | 150 |
| Graball | 22 | Sumner County | 126 |
| Bransford | 23 | Macon County | 124 |