City | Rank | County | 2024 Pop. |
|---|---|---|---|
| Lexington | 1 | Dawson County | 10,866 |
| Holdrege | 2 | Phelps County | 5,549 |
| Cozad | 3 | Dawson County | 3,916 |
| Gothenburg | 4 | Dawson County | 3,414 |
| Cambridge | 5 | Furnas County | 1,074 |
| Alma | 6 | Harlan County | 1,026 |
| Elm Creek | 7 | Buffalo County | 976 |
| Arapahoe | 8 | Furnas County | 951 |
| Bertrand | 9 | Phelps County | 714 |
| Oxford | 10 | Furnas County | 696 |
| Elwood | 11 | Gosper County | 629 |
| Overton | 12 | Dawson County | 586 |
| Beaver City | 13 | Furnas County | 528 |
| Eustis | 14 | Frontier County | 402 |
| Loomis | 15 | Phelps County | 391 |
| Orleans | 16 | Harlan County | 330 |
| Sumner | 17 | Dawson County | 241 |
| Holbrook | 18 | Furnas County | 198 |
| Farnam | 19 | Dawson County | 175 |
| Stamford | 20 | Harlan County | 155 |
| Miller | 21 | Buffalo County | 126 |
| Edison | 22 | Furnas County | 109 |
| Atlanta | 23 | Phelps County | 106 |
| Eddyville | 24 | Dawson County | 85 |
| Wilsonville | 25 | Furnas County | 73 |
| Smithfield | 26 | Gosper County | 58 |
| Lebanon | 27 | Red Willow County | 45 |
| Hendley | 28 | Furnas County | 22 |