City | Rank | County | 2024 Pop. |
|---|---|---|---|
| Fort Leonard Wood | 1 | Pulaski County | 15,707 |
| Lebanon | 2 | Laclede County | 15,564 |
| Marshfield | 3 | Webster County | 7,963 |
| St. Robert | 4 | Pulaski County | 5,521 |
| Waynesville | 5 | Pulaski County | 5,463 |
| Mountain Grove | 6 | Wright County | 4,753 |
| Ava | 7 | Douglas County | 3,242 |
| Willow Springs | 8 | Howell County | 2,231 |
| Houston | 9 | Texas County | 2,219 |
| Cabool | 10 | Texas County | 2,051 |
| Seymour | 11 | Webster County | 1,929 |
| Richland | 12 | Pulaski County | 1,737 |
| Mansfield | 13 | Wright County | 1,313 |
| Fordland | 14 | Webster County | 814 |
| Gainesville | 15 | Ozark County | 786 |
| Conway | 16 | Laclede County | 736 |
| Hartville | 17 | Wright County | 635 |
| Norwood | 18 | Wright County | 623 |
| Niangua | 19 | Webster County | 412 |
| Diggins | 20 | Webster County | 316 |
| Stoutland | 21 | Laclede County | 215 |
| Theodosia | 22 | Ozark County | 205 |
| Bakersfield | 23 | Ozark County | 198 |
| Sundown | 24 | Marion County | 186 |
| Phillipsburg | 25 | Laclede County | 166 |
| Pontiac | 26 | Ozark County | 112 |
| Bennett Springs | 27 | Camden County | 106 |
| Wasola | 28 | Ozark County | 106 |
| Plato | 29 | Texas County | 91 |
| Grovespring | 30 | Wright County | 75 |
| South Fork | 31 | Howell County | 64 |
| Evergreen | 32 | Camden County | 17 |