City | Rank | County | 2024 Pop. |
|---|---|---|---|
| Shepherdsville | 1 | Bullitt County | 14,839 |
| Bardstown | 2 | Nelson County | 13,916 |
| Campbellsville | 3 | Taylor County | 11,723 |
| Harrodsburg | 4 | Mercer County | 9,269 |
| Lebanon | 5 | Marion County | 6,593 |
| Columbia | 6 | Adair County | 4,894 |
| Hodgenville | 7 | Larue County | 3,333 |
| Springfield | 8 | Washington County | 2,968 |
| Russell Springs | 9 | Russell County | 2,816 |
| Junction City | 10 | Boyle County | 2,294 |
| Greensburg | 11 | Green County | 2,250 |
| Liberty | 12 | Casey County | 1,995 |
| Lebanon Junction | 13 | Bullitt County | 1,809 |
| Bloomfield | 14 | Nelson County | 981 |
| New Haven | 15 | Nelson County | 818 |
| Perryville | 16 | Boyle County | 792 |
| Magnolia | 17 | Larue County | 725 |
| Loretto | 18 | Marion County | 713 |
| Chaplin | 19 | Nelson County | 634 |
| Buffalo | 20 | Larue County | 576 |
| Hustonville | 21 | Lincoln County | 402 |
| Summersville | 22 | Green County | 332 |
| Willisburg | 23 | Washington County | 301 |
| Bradfordsville | 24 | Marion County | 274 |
| Hardyville | 25 | Hart County | 272 |
| Salvisa | 26 | Mercer County | 231 |
| Mackville | 27 | Washington County | 207 |
| Boston | 28 | Nelson County | 206 |
| Raywick | 29 | Marion County | 155 |
| St. Mary | 30 | Marion County | 144 |
| Fairfield | 31 | Nelson County | 133 |
| New Hope | 32 | Nelson County | 19 |