Note: As defined by the US Census Bureau, a city's official boundaries often extend miles beyond the city itself and may even cross county lines. As such, several thousand US cities officially exist in two or more counties (and therefore appear on multiple counties' lists of cities.)
City | Rank | 2024 Pop. | 2020 Pop. | Change | Density (/mi²) | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Hamilton township | 1 | 11,574 | 11,384 | 0.56% | 326 | 35.5 | Township |
| Hamilton township | 2 | 8,427 | 8,594 | -0.77% | 220 | 38.3 | Township |
| Punxsutawney | 3 | 5,562 | 5,766 | -0.91% | 1,652 | 3.4 | Borough |
| Brookville | 4 | 3,894 | 3,990 | -0.61% | 1,238 | 3.1 | Borough |
| Hamilton township | 5 | 2,783 | 2,711 | 0.83% | 206 | 13.5 | Township |
| Winslow township | 6 | 2,489 | 2,545 | -0.28% | 55 | 45.1 | Township |
| Reynoldsville | 7 | 2,479 | 2,547 | -0.68% | 1,786 | 1.4 | Borough |
| Snyder township | 8 | 2,312 | 2,366 | -0.3% | 56 | 41.1 | Township |
| Brockway | 9 | 2,224 | 2,279 | -0.63% | 1,877 | 1.2 | Borough |
| Coolspring township | 10 | 2,067 | 2,112 | -0.67% | 110 | 18.8 | Township |
| Bell township | 11 | 1,966 | 2,009 | -0.3% | 106 | 18.6 | Township |
| Henderson township | 12 | 1,903 | 1,940 | -0.16% | 87 | 21.9 | Township |
| Young township | 13 | 1,673 | 1,715 | -0.36% | 108 | 15.4 | Township |
| Warsaw township | 14 | 1,336 | 1,366 | -0.3% | 26 | 50.9 | Township |
| Pine Creek township | 15 | 1,303 | 1,325 | -0.23% | 46 | 28.3 | Township |
| Perry township | 16 | 1,218 | 1,248 | -0.33% | 43 | 28.4 | Township |
| Rose township | 17 | 1,129 | 1,153 | -0.27% | 59 | 19.1 | Township |
| McCalmont township | 18 | 1,111 | 1,136 | -0.36% | 42 | 26.2 | Township |
| Sykesville | 19 | 1,081 | 1,112 | -0.73% | 683 | 1.6 | Borough |
| Falls Creek | 20 | 1,007 | 1,033 | -0.69% | 1,080 | 0.9 | Borough |
| Big Run | 21 | 629 | 644 | -0.63% | 910 | 0.7 | Borough |
| Summerville | 22 | 497 | 511 | -0.8% | 835 | 0.6 | Borough |
| Corsica | 23 | 311 | 319 | -0.64% | 687 | 0.5 | Borough |
| Timblin | 24 | 140 | 144 | -0.71% | 155 | 0.9 | Borough |
| Worthville | 25 | 70 | 73 | -1.41% | 391 | 0.2 | Borough |
Note: City markers shown outside the county boundary mean that a part of the city extends into this county, but is primarily located in an adjacent county.
Hamilton township is the largest city in Jefferson County.
Worthville is the smallest city in Jefferson County.
There are 25 cities in Jefferson County, Pennsylvania.