Note: As defined by the US Census Bureau, a city's official boundaries often extend miles beyond the city itself and may even cross county lines. As such, several thousand US cities officially exist in two or more counties (and therefore appear on multiple counties' lists of cities.)
City | Rank | 2024 Pop. | 2020 Pop. | Change | Density (/mi²) | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Steubenville | 1 | 18,035 | 18,149 | -0.16% | 1,712 | 10.5 | City |
| Toronto | 2 | 5,154 | 5,297 | -0.69% | 2,471 | 2.1 | City |
| Wintersville | 3 | 3,646 | 3,766 | -0.82% | 1,167 | 3.1 | Village |
| Dillonvale | 4 | 3,290 | 3,411 | -0.39% | 3,688 | 0.9 | CDP |
| Mingo Junction | 5 | 3,228 | 3,342 | -0.89% | 1,200 | 2.7 | Village |
| Tiltonsville | 6 | 1,214 | 1,256 | -0.9% | 2,317 | 0.5 | Village |
| Brilliant | 7 | 999 | 1,302 | -3.38% | 572 | 1.7 | CDP |
| Yorkville | 8 | 933 | 967 | -0.95% | 1,576 | 0.6 | Village |
| Adena | 9 | 640 | 664 | -0.93% | 1,192 | 0.5 | Village |
| Dillonvale | 10 | 594 | 614 | -0.83% | 1,539 | 0.4 | Village |
| Bergholz | 11 | 524 | 542 | -0.95% | 931 | 0.6 | Village |
| Amsterdam | 12 | 422 | 437 | -0.94% | 1,319 | 0.3 | Village |
| Richmond | 13 | 397 | 411 | -1% | 718 | 0.6 | Village |
| Rayland | 14 | 379 | 393 | -1.04% | 801 | 0.5 | Village |
| Mount Pleasant | 15 | 378 | 392 | -1.05% | 1,482 | 0.3 | Village |
| Irondale | 16 | 313 | 325 | -0.95% | 216 | 1.4 | Village |
| Stratton | 17 | 258 | 264 | -0.77% | 480 | 0.5 | Village |
| Empire | 18 | 224 | 228 | -0.44% | 747 | 0.3 | Village |
| New Alexandria | 19 | 221 | 231 | -1.34% | 602 | 0.4 | Village |
| Bloomingdale | 20 | 134 | 138 | -0.74% | 1,523 | 0.1 | Village |
| East Springfield | 21 | 130 | 263 | 8.33% | 317 | 0.4 | CDP |
| Pottery Addition | 22 | 68 | 139 | -19.05% | 80 | 0.8 | CDP |
Note: City markers shown outside the county boundary mean that a part of the city extends into this county, but is primarily located in an adjacent county.
Steubenville is the largest city in Jefferson County.
Pottery Addition is the smallest city in Jefferson County.
There are 22 cities in Jefferson County, Ohio.