3 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Nicholasville | 1 | 33,283 | 31,137 | 1.31% | 2,219 | 15 | City |
| Wilmore | 2 | 6,169 | 6,006 | 0.54% | 1,240 | 4.98 | City |
| Keene | 3 | 106 | 100 | 0.95% | 862 | 0.12 | City |
Nicholasville is the largest city in Jessamine County, with a population of 33,283.
Keene is the smallest city in Jessamine County.
There are 3 cities in Jessamine County, Kentucky.