5 cities
| City | Rank | 2025 Pop. ↓ | 2020 Pop. | Change | Density | Area (mi²) | Type |
|---|---|---|---|---|---|---|---|
| Yulee | 1 | 14,347 | 13,346 | 1.34% | 619 | 23.16 | CDP |
| Fernandina Beach | 2 | 14,017 | 13,092 | 1.34% | 1,184 | 11.84 | City |
| Nassau Village-Ratliff | 3 | 4,328 | 4,789 | -1.55% | 295 | 14.67 | CDP |
| Hilliard | 4 | 3,116 | 2,963 | 1% | 566 | 5.51 | Town |
| Callahan | 5 | 1,972 | 1,537 | 4.62% | 1,091 | 1.81 | Town |
Yulee is the largest city in Nassau County, with a population of 14,347.
Callahan is the smallest city in Nassau County.
There are 5 cities in Nassau County, Florida.