There are 41 cities in Middlesex County, Massachusetts.
Note: As defined by the US Census Bureau, a city's official boundaries often extend miles beyond the city itself and may even cross county lines. As such, several thousand US cities officially exist in two or more counties (and therefore appear on multiple counties' lists of cities.)
City | Rank | State | 2024 Population | 2020 Census | Annual Change | Density (mi²) | Area (mi²) |
|---|---|---|---|---|---|---|---|
| Boston | 1 | Massachusetts | 629,842 | 671,570 | -1.63% | 13,029 | 48.34 |
| Cambridge | 2 | Massachusetts | 118,627 | 118,349 | 0.06% | 18,544 | 6.40 |
| Lowell | 3 | Massachusetts | 111,850 | 115,366 | -0.78% | 8,218 | 13.61 |
| Newton | 4 | Massachusetts | 86,003 | 88,759 | -0.8% | 4,824 | 17.83 |
| Somerville | 5 | Massachusetts | 78,710 | 80,814 | -0.66% | 19,090 | 4.12 |
| Medford | 6 | Massachusetts | 70,384 | 60,414 | 3.67% | 8,692 | 8.10 |
| Framingham | 7 | Massachusetts | 69,790 | 72,136 | -0.83% | 2,787 | 25.04 |
| Malden | 8 | Massachusetts | 63,413 | 66,011 | -1.01% | 12,569 | 5.04 |
| Waltham | 9 | Massachusetts | 63,084 | 65,046 | -0.77% | 4,950 | 12.74 |
| Brookline | 10 | Massachusetts | 62,854 | 59,223 | 0.12% | 9,298 | 6.76 |
| Methuen Town | 11 | Massachusetts | 53,497 | 52,985 | 0.24% | 2,408 | 22.21 |
| Everett | 12 | Massachusetts | 49,783 | 48,917 | 0.44% | 14,573 | 3.42 |
| Arlington | 13 | Massachusetts | 45,628 | 45,379 | -0.3% | 8,874 | 5.14 |
| Woburn | 14 | Massachusetts | 41,705 | 40,791 | 0.55% | 3,297 | 12.65 |
| Marlborough | 15 | Massachusetts | 40,288 | 41,654 | -0.84% | 1,931 | 20.86 |
| Chelsea | 16 | Massachusetts | 36,691 | 40,583 | -2.58% | 16,535 | 2.22 |
| Watertown Town | 17 | Massachusetts | 34,713 | 35,331 | -0.44% | 8,687 | 4 |
| Lexington | 18 | Massachusetts | 34,193 | 33,304 | -0.04% | 2,081 | 16.43 |
| Melrose | 19 | Massachusetts | 28,602 | 29,708 | -0.96% | 6,108 | 4.68 |
| Wakefield | 20 | Massachusetts | 27,067 | 27,041 | 0.03% | 3,661 | 7.39 |
| Belmont | 21 | Massachusetts | 26,879 | 26,158 | -0.22% | 5,784 | 4.65 |
| Burlington | 22 | Massachusetts | 26,039 | 28,077 | -0.25% | 2,216 | 11.75 |
| Reading | 23 | Massachusetts | 25,279 | 25,236 | -0.27% | 2,534 | 9.98 |
| Wilmington | 24 | Massachusetts | 22,992 | 23,390 | -0.43% | 1,355 | 16.97 |
| Winchester | 25 | Massachusetts | 22,858 | 22,760 | 0.11% | 3,789 | 6.03 |
| Stoneham | 26 | Massachusetts | 22,822 | 23,625 | -0.37% | 3,794 | 6.02 |
| Hudson | 27 | Massachusetts | 15,598 | 15,222 | 0.33% | 2,715 | 5.75 |
| Maynard | 28 | Massachusetts | 10,609 | 10,900 | -0.29% | 2,036 | 5.21 |
| Cochituate | 29 | Massachusetts | 6,879 | 6,730 | -0.42% | 1,806 | 3.81 |
| Pinehurst | 30 | Massachusetts | 5,972 | 6,997 | -3.66% | 1,601 | 3.73 |
| West Concord | 31 | Massachusetts | 5,606 | 7,294 | -7% | 1,661 | 3.38 |
| Hopkinton | 32 | Massachusetts | 2,760 | 3,864 | -8.88% | 1,802 | 1.53 |
| Ayer | 33 | Massachusetts | 2,751 | 2,551 | -3.81% | 2,075 | 1.33 |
| Littleton Common | 34 | Massachusetts | 2,538 | 3,370 | -6.14% | 717 | 3.54 |
| East Pepperell | 35 | Massachusetts | 2,082 | 1,916 | 2.06% | 1,470 | 1.42 |
| Pepperell | 36 | Massachusetts | 2,039 | 2,685 | -3.91% | 985 | 2.07 |
| Groton | 37 | Massachusetts | 1,817 | 1,127 | 10.52% | 1,087 | 1.67 |
| Devens | 38 | Massachusetts | 1,519 | 2,007 | -7.43% | 224 | 6.78 |
| Hanscom AFB | 39 | Massachusetts | 1,518 | 2,119 | -8.06% | 1,702 | 0.89 |
| Townsend | 40 | Massachusetts | 1,225 | 1,276 | 3.99% | 723 | 1.69 |
| Shirley | 41 | Massachusetts | 1,002 | 1,716 | -15.09% | 760 | 1.32 |
Note: City markers shown outside the county boundary mean that a part of the city extends into this county, but is primarily located in an adjacent county.
Boston is the largest city in Middlesex County.
Shirley is the smallest city in Middlesex County.
There are 41 cities in Middlesex County, Massachusetts.