9 cities
| City | County | 2025 Pop. ↓ |
|---|---|---|
| Johnson City | Washington County | 74,814 |
| Jackson | Madison County | 68,307 |
| Jefferson City | Jefferson County | 8,983 |
| Jonesborough | Washington County | 6,858 |
| Jasper | Marion County | 3,624 |
| Jacksboro | Campbell County | 2,265 |
| Jellico | Campbell County | 2,120 |
| Jamestown | Fentress County | 2,114 |
| John Sevier | Knox County | 1,041 |