14 cities
| City | County | 2025 Pop. ↓ |
|---|---|---|
| Joplin | Jasper County | 53,956 |
| Jefferson City | Cole County | 42,570 |
| Jackson | Cape Girardeau County | 15,939 |
| Jennings | St. Louis County | 12,792 |
| Jasper | Jasper County | 856 |
| Jonesburg | Montgomery County | 803 |
| Jamesport | Daviess County | 571 |
| Josephville | St. Charles County | 561 |
| Jane | McDonald County | 408 |
| Jamestown | Moniteau County | 332 |
| Junction City | Madison County | 281 |
| Jerico Springs | Cedar County | 169 |
| Jacksonville | Randolph County | 120 |
| Jameson | Daviess County | 71 |