33 cities
| City | County | 2025 Pop. ↓ |
|---|---|---|
| Eldridge | Scott County | 6,925 |
| Estherville | Emmet County | 5,792 |
| Evansdale | Black Hawk County | 4,445 |
| Emmetsburg | Palo Alto County | 3,574 |
| Eagle Grove | Wright County | 3,542 |
| Eldora | Hardin County | 2,547 |
| Ely | Linn County | 2,336 |
| Epworth | Dubuque County | 2,026 |
| Earlham | Madison County | 1,412 |
| Elkader | Clayton County | 1,186 |
| Elkhart | Polk County | 1,140 |
| Elk Run Heights | Black Hawk County | 1,047 |
| Eddyville | Wapello County | 951 |
| Edgewood | Clayton County | 911 |
| Eldon | Wapello County | 771 |
| Exira | Audubon County | 745 |
| Earlville | Delaware County | 717 |
| Essex | Page County | 712 |
| Elgin | Fayette County | 666 |
| Elk Horn | Shelby County | 651 |
| Early | Sac County | 575 |
| Everly | Clay County | 571 |
| Ellsworth | Hamilton County | 482 |
| Elma | Howard County | 479 |
| Emerson | Mills County | 401 |
| Earling | Shelby County | 392 |
| Elliott | Montgomery County | 325 |
| Elberon | Tama County | 177 |
| Exline | Appanoose County | 156 |
| East Peru | Clarke County | 120 |
| East Amana | Iowa County | 44 |
| Elkport | Clayton County | 28 |
| Ellston | Ringgold County | 21 |