23 cities
| City | County | 2025 Pop. ↓ |
|---|---|---|
| Estero | Lee County | 38,493 |
| Egypt Lake-Leto | Hillsborough County | 36,180 |
| East Lake | Pinellas County | 34,296 |
| East Lake-Orient Park | Hillsborough County | 30,865 |
| Eustis | Lake County | 25,281 |
| Edgewater | Volusia County | 23,964 |
| Ensley | Escambia County | 22,534 |
| Englewood | Charlotte County | 20,507 |
| East Milton | Santa Rosa County | 14,699 |
| Elfers | Pasco County | 13,124 |
| Ellenton | Manatee County | 5,670 |
| Eagle Lake | Polk County | 4,907 |
| Eglin AFB | Okaloosa County | 2,880 |
| Edgewood | Orange County | 2,774 |
| Eastpoint | Franklin County | 2,696 |
| Eatonville | Orange County | 2,247 |
| East Bronson | Alachua County | 1,919 |
| El Portal | Miami-Dade County | 1,844 |
| East Palatka | Putnam County | 1,418 |
| East Williston | Levy County | 669 |
| Everglades | Collier County | 402 |
| Esto | Holmes County | 358 |
| Ebro | Washington County | 248 |