28 cities
| City | County | 2025 Pop. ↓ |
|---|---|---|
| Davie | Broward County | 108,978 |
| Deltona | Volusia County | 101,869 |
| Daytona Beach | Volusia County | 89,290 |
| Deerfield Beach | Broward County | 87,699 |
| Doral | Miami-Dade County | 81,460 |
| Delray Beach | Palm Beach County | 68,019 |
| DeLand | Volusia County | 46,206 |
| Dunedin | Pinellas County | 35,839 |
| Dania Beach | Broward County | 32,039 |
| DeBary | Volusia County | 23,809 |
| Davenport | Polk County | 18,632 |
| Destin | Okaloosa County | 14,354 |
| Doctor Phillips | Orange County | 12,602 |
| Dade City | Pasco County | 9,615 |
| DeFuniak Springs | Walton County | 7,295 |
| Dundee | Polk County | 6,557 |
| Daytona Beach Shores | Volusia County | 5,241 |
| Dover | Hillsborough County | 3,133 |
| Dade City North | Hernando County | 2,920 |
| De Leon Springs | Volusia County | 2,778 |
| Dunnellon | Citrus County | 2,021 |
| DeLand Southwest | Lake County | 1,278 |
| Desoto Lakes | Sarasota County | 1,261 |
| Desoto Acres | Sarasota County | 881 |
| Duck Key | Monroe County | 758 |
| Day | Lafayette County | 219 |
| Dickerson City | Santa Rosa County | 145 |
| Dixonville | Santa Rosa County | 14 |