30 cities
| City | County | 2025 Pop. ↓ |
|---|---|---|
| Alafaya | Orange County | 94,886 |
| Apopka | Orange County | 61,907 |
| Altamonte Springs | Seminole County | 44,645 |
| Aventura | Miami-Dade County | 37,873 |
| Apollo Beach | Hillsborough County | 29,541 |
| Auburndale | Polk County | 22,854 |
| Azalea Park | Orange County | 16,891 |
| Asbury Lake | Clay County | 13,469 |
| Atlantic Beach | Duval County | 12,972 |
| Alachua | Alachua County | 10,898 |
| Avon Park | Highlands County | 10,588 |
| Arcadia | DeSoto County | 8,116 |
| Ave Maria | Collier County | 7,369 |
| Alturas | Polk County | 3,279 |
| Alva | Lee County | 3,084 |
| Apalachicola | Franklin County | 2,462 |
| Astatula | Lake County | 2,155 |
| Atlantis | Palm Beach County | 2,098 |
| Astor | Lake County | 1,447 |
| Archer | Alachua County | 1,161 |
| Allentown | Santa Rosa County | 1,064 |
| Anna Maria | Manatee County | 1,033 |
| Andrews | Levy County | 980 |
| Avalon | Santa Rosa County | 801 |
| Alford | Jackson County | 534 |
| Altha | Calhoun County | 498 |
| Acacia Villas | Palm Beach County | 347 |
| Aucilla | Jefferson County | 199 |
| Aripeka | Pasco County | 154 |
| Altoona | Lake County | 32 |