22 cities
| City | Rank | County | 2025 Pop. ↓ |
|---|---|---|---|
| Andalusia | 1 | Covington County | 8,836 |
| Atmore | 2 | Escambia County | 8,272 |
| Opp | 3 | Covington County | 6,763 |
| Brewton | 4 | Escambia County | 5,109 |
| Evergreen | 5 | Conecuh County | 3,260 |
| East Brewton | 6 | Conecuh County | 2,226 |
| Florala | 7 | Covington County | 1,943 |
| Flomaton | 8 | Escambia County | 1,444 |
| Babbie | 9 | Conecuh County | 650 |
| River Falls | 10 | Covington County | 499 |
| Castleberry | 11 | Conecuh County | 456 |
| Lockhart | 12 | Covington County | 448 |
| Red Level | 13 | Covington County | 437 |
| Carolina | 14 | Covington County | 294 |
| Sanford | 15 | Covington County | 270 |
| Heath | 16 | Conecuh County | 245 |
| Repton | 17 | Conecuh County | 223 |
| Horn Hill | 18 | Covington County | 210 |
| Gantt | 19 | Covington County | 205 |
| Riverview | 20 | Conecuh County | 165 |
| Pollard | 21 | Escambia County | 125 |
| Libertyville | 22 | Covington County | 108 |
Covington County, Escambia County, and Conecuh County are the counties generally considered part of South Alabama.